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Image Search Results
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
Article Title: Power-free microchip immunoassay of PSA in human serum for point-of-care testing.
doi: 10.2116/analsci.27.237
Figure Lengend Snippet: Fig. 2 Time course of the fluorescent intensity of the microfluidic immunoassay. The 0 min of amplification time in the horizontal axis is defined as the time from which both F-SA and b-anti-SA start flowing into the microchannel. PSA was diluted with PBS containing 1% BSA (a-1 and a-2) or human female serum (b-1 and b-2). Each data point is the average of four measurements obtained by four independent experiments. Additionally, fluorescence was obtained from LFDA (a-1 and b-1) or from no amplification (a-2 and b-2). The concentrations of PSA: blank (▼), 4.0 ng/mL (△), 100 ng/mL (■), 1.0 μg/mL (×), and 10 μg/mL (◣).
Article Snippet: Sample solutions of PSA were created by diluting
Techniques: Amplification, Fluorescence
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
Article Title: Power-free microchip immunoassay of PSA in human serum for point-of-care testing.
doi: 10.2116/analsci.27.237
Figure Lengend Snippet: Fig. 3 Calibration curve of PSA diluted in PBS (a-1 and a-2) or human female serum (b-1 and b-2). The error bars represent one standard deviation of four independent experiments. (a-1 or b-1) The curves were obtained from LFDA and the amplification time was 7.0 min. Insets magnify the graphs of the low concentration range. (a-2 and b-2) Calibration curve of PSA without amplification as control experiments. The time of detection was 4.0 min after the start of flow of F-SA.
Article Snippet: Sample solutions of PSA were created by diluting
Techniques: Standard Deviation, Amplification, Concentration Assay, Control
Journal: EMBO Molecular Medicine
Article Title: A novel direct activator of AMPK inhibits prostate cancer growth by blocking lipogenesis
doi: 10.1002/emmm.201302734
Figure Lengend Snippet: In vivo effects of AMPK activation. Average tumor volume of LNCaP xenografts during 14-day treatment with MT 63–78 (30 mg/kg, i.p.). Experiments were performed twice for a total number of 17 mice under treatment and 15 controls. Results are expressed as n fold the mean initial volume (equal to 1) ± s.e.m. (* P = 0.049, end of treatment, Unpaired t -test). Representative image of treated and control mice (with similar pre-treatment tumor volume) and their excised tumors at sacrifice. Correspondent western blot analysis of key proteins belonging to lipogenic and mTORC1 pathways is shown. Densitometric analysis of phosphorylated ACC and Raptor in tumor homogenates of all treated mice ( n = 16) and controls ( n = 15). One treated mouse was not included in the analysis because frozen material was not available. Highlighted (black circle) are the two tumors with the highest ACC phosphorylation and volume reduction. Results are expressed in arbitrary units, after normalization to Vinculin. Unpaired t -test and Wilcoxon Mann–Whitney t -test were performed. Significant P values are reported on the graphs. Average tumor volume of LNCaP xenografts during 21-day treatment with MT 63–78 (60 mg/kg, i.p., five mice) and vehicle (six mice). Results are expressed as n fold the mean initial volume (equal to 1) ± s.e.m. (** P = 0.004, end of treatment, Wilcoxon Mann–Whitney test). One mouse from treated group was removed from the study since biochemical analysis showed that the drug for unknown reasons did not penentrate in the tumor. Densitometric analysis of phosphorylated ACC and Raptor performed as in C. Unpaired t -tests were performed. Significant P values are reported on the graphs. Serum PSA levels measured at the end of the 21-day treatment with MT 63–78 (60 mg/kg, i.p.) in five treated and five control mice. Results are expressed as mean ± s.e.m. ELISA was performed twice (each sample in duplicate) with similar results. A representative experiment is shown. Serum from one control mouse was not available. Unpaired t -test was performed ( P = 0.27). PIN incidence in FASN-Tg α2 +/+ ( n = 34), FASN-Tg/AMPK α2 +/− ( n = 28), FASN-Tg/AMPK α2 −/− ( n = 7) age-matched mice. Logistic regression P = 0.077, with post hoc calculated power of 0.44. Source data are available for this figure.
Article Snippet: Serum PSA levels were measured using Total Prostate-Specific
Techniques: In Vivo, Activation Assay, Control, Western Blot, Phospho-proteomics, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay
Journal: Oncology Letters
Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma
doi: 10.3892/ol.2018.8468
Figure Lengend Snippet: SNPs located in exons.
Article Snippet:
Techniques:
Journal: Oncology Letters
Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma
doi: 10.3892/ol.2018.8468
Figure Lengend Snippet: Allele distribution of the 12 selected SNPs.
Article Snippet:
Techniques: Control
Journal: Oncology Letters
Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma
doi: 10.3892/ol.2018.8468
Figure Lengend Snippet: The rs2294008 polymorphism in PSCA . OSCC, oral squamous cell carcinoma; PSCA, prostate stem cell antigen.
Article Snippet:
Techniques:
Journal: Oncology Letters
Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma
doi: 10.3892/ol.2018.8468
Figure Lengend Snippet: Genotype distribution of the 8 SNPs.
Article Snippet:
Techniques: Control
Journal: Oncology Letters
Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma
doi: 10.3892/ol.2018.8468
Figure Lengend Snippet: PSCA expression pattern in eight cancer and two normal cell lines. OSCC, oral squamous cell carcinoma; PSCA, prostate stem cell antigen.
Article Snippet:
Techniques: Expressing
Journal: Oncology Letters
Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma
doi: 10.3892/ol.2018.8468
Figure Lengend Snippet: PSCA expression in OSCC and normal serum.
Article Snippet:
Techniques: Expressing
Journal: Oncology Letters
Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma
doi: 10.3892/ol.2018.8468
Figure Lengend Snippet: PSCA protein expression in OSCC and normal tissues.
Article Snippet:
Techniques: Expressing, Enzyme-linked Immunosorbent Assay
Journal: Oncology Letters
Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma
doi: 10.3892/ol.2018.8468
Figure Lengend Snippet: PSCA expression in OSCC tissues. (A and B) Representative images indicating PSCA expression in tissues from patients with OSCC and PSCA-positive serum (IRS=6; A, 100×; B, 200×). (C and D) PSCA expression in tissues from patients with OSCC and PSCA-negative serum (IRS=2; C, ×100; D, ×200). OSCC, oral squamous cell carcinoma; PSCA, prostate stem cell antigen.
Article Snippet:
Techniques: Expressing
Journal: Oncology Letters
Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma
doi: 10.3892/ol.2018.8468
Figure Lengend Snippet: PSCA expression in normal epithelium. Representative images from normal tissue with an IRS=0 (A, ×100; B, ×200). OSCC, oral squamous cell carcinoma; PSCA, prostate stem cell antigen.
Article Snippet:
Techniques: Expressing
Journal: Oncology Letters
Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma
doi: 10.3892/ol.2018.8468
Figure Lengend Snippet: PSCA expression in OSCC epithelium with different genotypes. (A and B) Representative images indicating PSCA expression in tissues from OSCC patients presenting the CC genotype (A, ×100; B, ×200). (C and D) Representative images indicating PSCA expression in OSCC patients presenting the CT genotype (C, ×100; D, ×200). Representative images indicating PSCA expression in tissues from OSCC patients presenting the TT genotype (E, ×100; F, ×200). OSCC, oral squamous cell carcinoma; PSCA, prostate stem cell antigen.
Article Snippet:
Techniques: Expressing